Aim: In this study a questionnaire was developed to objectify and quantify the effects of UV light exposure, and its practicability for use in a future cohort study was tested. In addition, the study served to screen and to evaluate willingness to take part in any future cohort study. Collective and Method: In a cross-sectional study, 101 voluntary subjects were asked by anamnesis questionnaire about UV exposure, constitutional factors, sunburn, family factors, drug anamnesis, etc. In addition, a dermatoscopic inspection of moles was carried out. Results: Among the 101 participants 19.6% belonged to the cockpit staff and 80.4% to the cabin staff. Comparing leisure activities during layover periods, holiday activities during the last 5 years and during childhood revealed that the interviewees had spent more hours per day on the beach during childhood than during both other periods. During all three periods the median daily hours spent in the mountains was the highest. 29 test persons showed noticeable skin changes. There was a low correspondence (p < 0.05) (Kappa = 0.38) between the number of naevi counted by the dermatologist and that counted by the study participants themselves. Conclusion: Constitutional and behavioural parameters can be determined using the newly developed questionnaire. It is recommended that screening for skin cancer be included in the scope of any future cohort study.